Potential resident bacterial microbiota in udder tissues of culled cows sampled in abattoir

While mammary gland tissues (MGTs) are difficult to sample without risks for cow's health or milk production, analysis used in routine assess dairy cow udder's health. This study aimed identify, quantify, compare the and MGTs microbiota of macroscopically healthy bovine glands (MG) order evaluate their degree similarity. We harvested 13 couples samples, originated from same quarter at culling. 16S rDNA Amplicon Sequencing was performed, showing Corynebacterium as main bacterial genus both types samples but generally found higher proportions than tissues. Species evenness while species richness samples. Beta diversity significantly different between matrices suggesting presence a resident cows time culling partially reflected by quarter. • Bacterial comparison Metagenetic amplicon sequencing V1-V3 regions. Microbiota show two distinct populations.